Zero order, Area under curve and dual wavelength Spectrophotometric methods has been developed for simultaneous estimation of Pantoprazole sodium and Levosulpiride in bulk drug and in pharmaceutical formulation. All these methods utilizes methanol as a solvent. In Zero order spectroscopy 291nm and 289nm were selected as analytical wavelength for determination of both the drugs simultaneously. In Area Under Curve the wavelength ranges of 283–298 nm and 282–297 nm were selected as analytical wavelength ranges for the determination of area of both the drugs in pure and in combined formulation. In Dual wavelength method, the absorbance difference was determined within 287nm and 295nm for the the determination of Pantoprazole, and absorbance difference within 286 nm and 292 nm for the determination of Leveosulpiride. The results of analysis of all methods have been validated statistically. Lower value of % RSD indicates that these methods were simple, rapid and accurate, hence can be used for routine simultaneous estimation of these drugs in formulations. The results were found to be within acceptance criteria according to ICH Q2 R1 guideline.
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